Title :
Ultrasonic characterization of cell culture medium in the temperature range from 25°C to 50°C
Author :
Lopez-Haro, S.A. ; Reyes, J.L. ; Vera, A. ; Leija, L.
Author_Institution :
Dept. of Electr. Eng., CINVESTAV-IPN, Mexico City, Mexico
Abstract :
The aim of this work was the ultrasonic characterization of the cell culture medium used for Madin-Darby canine kidney (MDCK) cells. To design and build a special container for the speed-of-sound (SOS) temperature dependence measurement of the cell culture media was necessary. Pulse-echo technique and a step reflector were used to obtain the temperature dependence of SOS results. Water and cell culture media SOS are compared; both liquids behavior show an upward tendency in the temperature range studied. Culture media SOS results were fitted by using both a first and a second order curve; second order curve had better correlation coefficient than the other one. Speed-of-sound varies from 1510 m/s at 25°C to 1540 m/s at 50°C.
Keywords :
biological tissues; biomedical measurement; biomedical ultrasonics; cellular biophysics; echo; kidney; Madin-Darby canine kidney cells; cell culture media; correlation coefficient; first order curve; pulse-echo technique; second order curve; speed-of-sound temperature dependence measurement; temperature 25 degC to 50 degC; time 1510 ms to 1540 ms; ultrasonic characterization; Acoustics; Media; Temperature dependence; Temperature measurement; Transducers; Ultrasonic imaging; Ultrasonic variables measurement; Cell culture Media; Speed-of-Sound; Temperature Dependence; Ultrasound;
Conference_Titel :
Health Care Exchanges (PAHCE), 2012 Pan American
Conference_Location :
Miami, FL
Print_ISBN :
978-1-4673-1135-9
DOI :
10.1109/PAHCE.2012.6233443
