Title :
Cortical blood flow imaging with a portable MEMS based 2-photon fluorescence microendoscope
Author :
Piyawattanametha, W. ; Solgaard, O. ; Schnitzer, M.J.
Author_Institution :
NECTEC, Pathumthani, Thailand
Abstract :
We present a portable microendoscope based on a microelectromechanical systems (MEMS) scanner, compound gradient refractive index micro-lenses and a photonics bandgap fiber (PBF). It overcomes the size (2.0 × 1.9 × 1.1 cm3) and weight (less than 3 grams) limitations of conventional two-photon fluorescence microscopy toward freely moving subjects. The microendoscope utilizes a PBF for laser excitation and large core fiber for fluorescence collection. We demonstrated cortical blood flow imaging in live mice with transverse (Δx) and axial resolutions (Δz) of 1.6 μm and 13.5 μm, respectively.
Keywords :
biomedical optical imaging; endoscopes; fluorescence; haemodynamics; 2-photon fluorescence microendoscope; compound gradient refractive index microlens; cortical blood flow imaging; microelectromechanical systems scanner; photonics bandgap fiber; portable MEMS; Fluorescence; Image resolution; Laser beams; Lenses; Micromechanical devices; Microscopy; 2-photon; MEMS; fluorescence; neuroscience; scanner;
Conference_Titel :
Solid-State Sensors, Actuators and Microsystems Conference (TRANSDUCERS), 2011 16th International
Conference_Location :
Beijing
Print_ISBN :
978-1-4577-0157-3
DOI :
10.1109/TRANSDUCERS.2011.5969746
