Quantification of membrane IHC stains through multi-spectral imaging

This work presents a fully automated approach to the quantification of the expression of antibodies in immunohistochemically stained tissue sections. Conventional RGB imaging was compared to multispectral imaging in the analysis of membrane stained tissue sections with high complexity, i.e. clustered and overlapping cells, and co-localization of stains. Preliminary results on more than 1700 cells suggest that multi-spectral imaging outperforms RGB imaging, particularly in complex and hard to segment regions.