Title :
A protein structure prediction and function identification
Author :
Mao, Rui ; Wu, Kuan
Author_Institution :
Coll. of Inf. Eng., Northwest A&F Univ., Yangling, China
Abstract :
To study the function of CNV p20 in planta, its bioinformation was analyzed using the software online. The CNV p20 protein sequence was aligned with TBSV p19, a strong RNA silencing suppressor (RSS). The secondary and crystal structure analysis of CNV p20 predicted that it was a RSS based on sequence similar with protein of known function. According to the result of the molecular biology experiments, CNV p20 was required for suppression of GFP transgene silencing. The function of CNV p20 was successfully predicted with its bioinformation and identified with the molecular biology analysis.
Keywords :
bioinformatics; crystal structure; molecular biophysics; molecular configurations; proteins; CNV p20 crystal structure analysis; CNV p20 protein sequence; CNV p20 secondary structure analysis; GFP transgene silencing suppression; RNA silencing suppressor; TBSV p19; bioinformation; protein function identification; protein structure prediction; Amino acids; Bioinformatics; Biological information theory; Educational institutions; Genomics; Information analysis; Information science; Protein engineering; Protein sequence; RNA; RNA silencing suppressor; function identification; protein structure; sequence alignment;
Conference_Titel :
Bioinformatics and Biomedical Technology (ICBBT), 2010 International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-6775-4
DOI :
10.1109/ICBBT.2010.5478974
