Title :
The cloning and characterization of genomic DNA encoding for the RPB5-mediating protein (RMP)
Author :
Gu, Junxia ; Wang, Xiaohua ; Zhuang, Wenzhuo ; Miao, Jingcheng ; Wei, Wenxiang
Author_Institution :
Dept. of Cellular & Mol. Biol., Suzhou Univ., Suzhou, China
Abstract :
Aims: To clone and characterize genomic gene encoding for RMP5-mediating protein (RMP). Methods: Genomic library of lambda phage was screened with cDNA probe of full RMP encoding amino acids from 1 to 508. Southern blot analysis, Mapping of restriction enzyme sites, PCR and DNA sequencing were applied for characterization of isolated clones. Results: the RMP genomic fragments were isolated, which encode for the middle and C-terminal of RMP, but lack N-terminal sequence. Restriction enzyme sites were mapped on the genomic DNA with sequence confirmed.
Keywords :
DNA; biochemistry; bioinformatics; cellular biophysics; encoding; enzymes; genetics; genomics; molecular biophysics; proteomics; DNA sequencing; PCR sequencing; RMP5-mediating protein; Southern blot analysis; amino acids; cDNA probe; genomic DNA; genomic gene encoding; isolated clones; lambda phage; restriction enzyme sites; Biochemistry; Bioinformatics; Cloning; DNA; Encoding; Genomics; Libraries; Probes; Proteins; Sequences; Genomic DNA; RMP; RPP5; cloning and characterization;
Conference_Titel :
Bioinformatics and Biomedical Technology (ICBBT), 2010 International Conference on
Conference_Location :
Chengdu
Print_ISBN :
978-1-4244-6775-4
DOI :
10.1109/ICBBT.2010.5478979
