Title :
3D single molecule tracking and superresolution microscopy using multifocal plane microscopy
Author :
Ram, Sripad ; Ward, Elizabeth Sally ; Ober, Raimund J.
Author_Institution :
Dept. of Immunology, Univ. of Texas Southwestern Med. Center, Dallas, TX, USA
Abstract :
The study of cellular processes in three-dimensions is severely limited by the lack of imaging methodologies that allow for fast 3D tracking of cellular events and 3D superresolution imaging of sub-cellular structures. We have developed a 3D imaging modality, multifocal plane microscopy (MUM), that provides a powerful approach for 3D single molecule tracking and 3D superresolution microscopy. Here we review the technical challenges associated with 3D single molecule localization that is fundamental to both 3D tracking and 3D superresolution, and discuss how MUM overcomes these problems.
Keywords :
biological techniques; cellular biophysics; maximum likelihood estimation; molecular biophysics; object tracking; optical microscopy; 3D single molecule localization; 3D single molecule tracking; 3D superresolution imaging; cellular processes; imaging modality; maximum likelihood estimation; multifocal plane microscopy; subcellular structures; superresolution microscopy; Accuracy; Image resolution; Microscopy; Optical microscopy; Optical variables measurement; Random access memory; 2D/3D localization accuracy; Cramer-Rao lower bound; Fisher information matrix; maximum likelihood estimator; resolution limits;
Conference_Titel :
Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on
Conference_Location :
Barcelona
Print_ISBN :
978-1-4577-1857-1
DOI :
10.1109/ISBI.2012.6235702
