• DocumentCode
    2570933
  • Title

    Optimal 3D single-molecule super-resolution microscopy with engineered point spread functions

  • Author

    Quirin, Sean ; Grover, Ginni ; Piestun, Rafael

  • Author_Institution
    Dept. of Electr., Comput., & Energy Eng., Univ. of Colorado at Boulder, Boulder, CO, USA
  • fYear
    2012
  • fDate
    2-5 May 2012
  • Firstpage
    926
  • Lastpage
    927
  • Abstract
    Three-dimensional single-molecule localization is a fundamental problem in biological and biophysical experiments such as super-resolution microscopy. Point spread function engineering accompanied by corresponding reconstruction procedures provides a unique approach to increase localization precision. Joint optical/digital design and image reconstruction are investigated.
  • Keywords
    bio-optics; biological techniques; optical transfer function; biological experiments; biophysical experiments; engineered point spread function; image reconstruction; joint optical-digital design; optimal 3D single molecule superresolution microscopy; Fluorescence; Image resolution; Microscopy; Optical microscopy; Photonics; USA Councils; Point spread function engineering; Single-molecule localization; Superresolution microscopy;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on
  • Conference_Location
    Barcelona
  • ISSN
    1945-7928
  • Print_ISBN
    978-1-4577-1857-1
  • Type

    conf

  • DOI
    10.1109/ISBI.2012.6235707
  • Filename
    6235707