Title :
Optimal 3D single-molecule super-resolution microscopy with engineered point spread functions
Author :
Quirin, Sean ; Grover, Ginni ; Piestun, Rafael
Author_Institution :
Dept. of Electr., Comput., & Energy Eng., Univ. of Colorado at Boulder, Boulder, CO, USA
Abstract :
Three-dimensional single-molecule localization is a fundamental problem in biological and biophysical experiments such as super-resolution microscopy. Point spread function engineering accompanied by corresponding reconstruction procedures provides a unique approach to increase localization precision. Joint optical/digital design and image reconstruction are investigated.
Keywords :
bio-optics; biological techniques; optical transfer function; biological experiments; biophysical experiments; engineered point spread function; image reconstruction; joint optical-digital design; optimal 3D single molecule superresolution microscopy; Fluorescence; Image resolution; Microscopy; Optical microscopy; Photonics; USA Councils; Point spread function engineering; Single-molecule localization; Superresolution microscopy;
Conference_Titel :
Biomedical Imaging (ISBI), 2012 9th IEEE International Symposium on
Conference_Location :
Barcelona
Print_ISBN :
978-1-4577-1857-1
DOI :
10.1109/ISBI.2012.6235707
