Phenoloxidase activity of phenol-degrading bacteria

Author

Han, Hong-Jun ; Fang, Fang ; Ma, Wen-Cheng ; Xun, Chun-Yan ; Wang, Wei ; Li, Hui-Qiang

Author_Institution

State Key Lab. of Urban Water Resources & Environ., Harbin Inst. of Technol., Harbin, China

fYear

2010

fDate

16-18 April 2010

Firstpage

23

Lastpage

26

Abstract

In order to study phenoloxidase (PO) activity of the phenol-degrading bacteria, the carbon source of isolation culture medium adopts artificial phenol mixture. Stenotrophomonas maltophilia K279a is isolated from coal chemical industry wastewater. The optimum conditions for PO production are phenol 1000mg/L, temperature 30-34°C and PH 7-7.5. The strain resists 980mg/L COD and 805mg/L phenols. The degradation rate of phenols reaches 97% in 48h. The results show that Stenotrophomonas maltophilia K279a can be applied to the aerobic process with high proportion of phenols.

Keywords

biochemistry; biological techniques; biotechnology; cellular biophysics; chemical technology; coal; enzymes; industrial waste; microorganisms; molecular biophysics; wastewater; Stenotrophomonas maltophilia K279a; aerobic process; artificial phenol mixture; coal chemical industry; degradation rate; isolation culture medium; phenol-degrading bacteria; phenoloxidase activity; wastewater; Biochemistry; Capacitive sensors; Chemical industry; Chemical technology; Degradation; Isolation technology; Microorganisms; Sequences; Wastewater; Water resources; bacteria; coal chemical industry wastewater; phenol; phenoloxidase;

fLanguage

English

Publisher

ieee

Conference_Titel

Bioinformatics and Biomedical Technology (ICBBT), 2010 International Conference on

Conference_Location

Chengdu

Print_ISBN

978-1-4244-6775-4

Type

conf

DOI

10.1109/ICBBT.2010.5479019

Filename

5479019