Title :
FEA for steady-state thermal performance of PCR thermal-cycler based on thermoelectric cooler
Author :
Huang, Jing ; Chen, Zhangwei ; Hu, Kewei
Author_Institution :
State Key Lab. of Fluid Power Transm. & Control, Zhejiang Univ., Hangzhou, China
Abstract :
Polymerase chain reaction (PCR) technology is a method for amplifying a specific target DNA sequence in vitro; thermoelectric cooler (TEC) is used in thermal-cycler system to implement periodic heating and cooling. Finite element models of multi-stage TEC and PCR thermal-cycler system based on TEC were established with ANSYS Workbench 12.0 thermal-electric coupled-field analysis elements. The steady-state thermal performances of TEC XLT2389 and thermal-cycler system with different driving currents were analyzed by this model. The simulation results indicated that the system steady-state temperature distribution was asymmetry for the energy loss on the sample block side surface; the system should work on a preferable driving current to achieve the optimal cooling ability.
Keywords :
DNA; biomedical engineering; finite element analysis; molecular biophysics; thermoelectric cooling; ANSYS Workbench 12.0; DNA sequence; PCR technology; PCR thermal cycler; TEC XLT2389 and; cooling; finite element model; multistage TEC; periodic heating; polymerase chain reaction; steady state thermal performance; thermoelectric cooler; Analytical models; Cooling; DNA; Finite element methods; Heating; Steady-state; Thermal analysis; PCR; finite elment analysis; thermal-cycler; thermoelectric cooler;
Conference_Titel :
Biomedical Engineering and Informatics (BMEI), 2011 4th International Conference on
Conference_Location :
Shanghai
Print_ISBN :
978-1-4244-9351-7
DOI :
10.1109/BMEI.2011.6098422
