In vitro anti-Influenza a virus activity of konjac oligo-glucomannan sulfate

Author

Huang, Hao ; Yang, Zhonghua ; Wang, Guanghui ; Chen, Jun

Author_Institution

Biol. Eng. Inst., Wu Han Univ. of Sci. & Technol., Wuhan, China

Volume

3

fYear

2011

fDate

15-17 Oct. 2011

Firstpage

1495

Lastpage

1501

Abstract

Glucomannan extracted from konjac was sulfated (konjac oligo-glucomannan sulfate, OGMS) to show selective anti-Influenza A virus activity in vitro using MDCK cell line as the host cell of Influenza A virus. OGMS straight inhibited the virus according to different groups and IAV-RNA Replication in MDCK cell by RT-PCR. The studies indicated that OGMS mainly acted as virus protein inhibitor and its IC₅₀ was 0.03125 mg/mL by Reed-Muench method; IAV-RNA Replication was inhibited as well. All these indicated that OGMS had evident in vitro anti-Influenza A virus activity.

Keywords

drugs; inhibitors; microorganisms; proteins; IAV-RNA Replication; IAV-RNA replication; IC50; MDCK cell line; OGMS; RT-PCR; Reed-Muench method; in vitro anti-Influenza A virus activity; konjac oligo-glucomannan sulfate; virus protein inhibitor; Educational institutions; In vitro; Influenza; Microscopy; Pathology; Plastics; RNA; In vitro; Influenza A virus; Konjac; Polysaccharide sulfate;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Informatics (BMEI), 2011 4th International Conference on

Conference_Location

Shanghai

Print_ISBN

978-1-4244-9351-7

Type

conf

DOI

10.1109/BMEI.2011.6098630

Filename

6098630