Title :
Modeling neurite outgrowth on laminin in vitro
Author :
Buettner, M., II ; Pittman, R.N.
Author_Institution :
Dept. of Chem. & Biochem. Eng., Rutgers Univ., Piscataway, NJ, USA
Abstract :
Experimental parameters were measured and incorporated into a mathematical model to describe axonal outgrowth on the extracellular matrix molecule, laminin, in vitro. To elucidate the role of laminin and other basement membrane components in axonal growth and guidance, a systematic analysis of the effects of these various molecules on outgrowth characteristics such as the rate and direction of growth is required. Ideal parameters for measuring these characteristics appear to be the root-mean-square speed, s, and the directional persistence time, P, of the growing axon tip. These quantities were measured for neurons obtained from the superior cervical ganglia of one day-old neonatal rats, mechanically dissociated into single cells and cultured in serum-free medium. To illustrate the potential effects of the observed concentration dependence of these parameters on axonal outgrowth, the ability of an axon to traverse a laminin substrate was examined using a hypothetical model for the axon trajectory based on the measured s and P
Keywords :
cellular transport and dynamics; neurophysiology; physiological models; axon trajectory; axonal outgrowth; basement membrane components; directional persistence time; experimental parameters; extracellular matrix molecule; growing axon tip; hypothetical model; in vitro; laminin; mathematical model; mechanically dissociated cells; neonatal rats; neurite outgrowth modeling; root-mean-square speed; serum-free medium; superior cervical ganglia; Biomembranes; Extracellular; In vitro; Mathematical model; Mechanical variables measurement; Nerve fibers; Neurons; Pediatrics; Time measurement; Velocity measurement;
Conference_Titel :
Bioengineering Conference, 1991., Proceedings of the 1991 IEEE Seventeenth Annual Northeast
Conference_Location :
Hartford, CT
Print_ISBN :
0-7803-0030-0
DOI :
10.1109/NEBC.1991.154629