Does Background Intensity Estimation Influence the Iterative Restoration of Microscope Images?

A new background estimation method is proposed to improve iterative restoration of microscope images. The method is based on a sequence of subtractions performed in the beginning of an iterative restoration procedure. A series of experiments using background intensity detection methods were carried out in order to analyse the influence of a correct estimation of the background. The restoration results as a function of the detected background value is discussed. The results indicates a strong influence of the estimate on the restoration of images. When compared to an optimal error-based method, the proposed method was faster and obtained a good approximation to the correct value.