Fluorescence nanoscopy: breaking the diffraction barrier by the RESOLFT concept

Author

Hell, Stefan W.

Author_Institution

Dept. of NanoBiophotonics, Max Planck Inst. for Biophys. Chem., Gottingen, Germany

fYear

2005

fDate

22-28 Oct. 2005

Firstpage

42

Abstract

In this lecture, principle of fundamentally breaking the diffraction barrier through reversible saturable optical (fluorescence) transitions (RESOLFT) is discussed. In these concepts, the diffraction barrier is broken by a saturated optical transition (depletion) between two states of a marker, whereby the transition is effected with an intensity distribution featuring one or more intensity minima (zero). The saturation level defines the size of the ultrasharp focal spot and/or the concomitantly enlarged bandwidth of the optical transfer function (OTF).

Keywords

fluorescence; light diffraction; nanotechnology; optical microscopy; optical saturation; optical transfer function; RESOLFT; diffraction barrier; fluorescence nanoscopy; optical transfer function; reversible saturable optical fluorescence transitions; saturated optical transition; Biomedical optical imaging; Chemistry; Fluorescence; Microscopy; Optical diffraction; Optical saturation; Optimized production technology; Spatial resolution; Stationary state; Stimulated emission;

fLanguage

English

Publisher

ieee

Conference_Titel

Lasers and Electro-Optics Society, 2005. LEOS 2005. The 18th Annual Meeting of the IEEE

Print_ISBN

0-7803-9217-5

Type

conf

DOI

10.1109/LEOS.2005.1547859

Filename

1547859