Title :
High-contrast detection of target organisms in highly autofluorescent backgrounds using time-resolved techniques
Author :
Piper, J.A. ; Connally, R.E. ; Jin, D.Y.
Author_Institution :
Macquarie Univ., Sydney, NSW, Australia
Abstract :
This paper reviews recent developments of practical lanthanide chelate probes, together with low-cost TRF microscopy and analogous continuous-flow TRF instrumentation, and demonstration of these techniques in detection of rare environmental pathogens.
Keywords :
bio-optics; europium; fluorescence; molecular biophysics; optical microscopy; terbium; time resolved spectroscopy; TRF microscopy; autofluorescence; lanthanide chelate probes; pathogens; target organism detection; time-resolved techniques; Detectors; Fluorescence; Instruments; Light emitting diodes; Microscopy; Molecular biophysics; Organisms; Pathogens; Probes; Sensor arrays;
Conference_Titel :
Lasers and Electro-Optics Society, 2005. LEOS 2005. The 18th Annual Meeting of the IEEE
Print_ISBN :
0-7803-9217-5
DOI :
10.1109/LEOS.2005.1547927
