High-speed single cell dispensing system

Author

Kawahara, Tomohiro ; Hirano, Tatsuhiko ; Feng, Lin ; Uvet, Huseyin ; Hagiwara, Masaya ; Yamanishi, Yoko ; Arai, Fumihito

Author_Institution

Grad. Sch. of Eng., Nagoya Univ., Nagoya, Japan

fYear

2011

fDate

6-9 Nov. 2011

Firstpage

472

Lastpage

474

Abstract

In this paper, we discuss the single cell dispensing system to increase both the speed and the success rate of single cell dispensing. Two pairs of capacitance sensors are placed in a biochip to detect the flow velocity of cells, and the air pressure is applied to eject cells by synchronizing the timing. Then, the system theoretically has a capability to eject 3 cells/sec with maximum flow velocity is 10 mm/sec. Finally, we succeeded in automatic dispensing of a single polystyrene bead (=100 μm) from a biochip to culture well atmosphere using developed cell ejection system with the success rate of 50 %. Furthermore, we also succeeded in single swine oocyte dispensing by developed system.

Keywords

biological techniques; biosensors; capacitance; cellular biophysics; lab-on-a-chip; air pressure; biochip; capacitance sensors; cell ejection system; flow velocity; high-speed single cell dispensing system; single polystyrene bead; single swine oocyte; Capacitance; Capacitive sensors; Microchannel; Solenoids; Substrates; Valves;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science (MHS), 2011 International Symposium on

Conference_Location

Nagoya

ISSN

Pending

Print_ISBN

978-1-4577-1360-6

Type

conf

DOI

10.1109/MHS.2011.6102235

Filename

6102235