2D-SPR affinity biosensing in 10 μm wells for multi-scale biosensing

We have been developing multi-scale affinity sensor which can be applied from 10 μm (single cell) to 1 mm ranges based on two-dimensional surface plasmon resonance (2D-SPR) imaging. But SPR sensitivity was drastically decreased in 10 μm wells because surface plasmon wave cannot propagate in such micro area. The use of shorter wavelength incident light might improve sensitivity of SPR sensing in micro wells. This paper described improvement of sensitivity and resolution of 2D-SPR imaging in 10 μm microwell array by using silver-based novel sensor chip and 505 nm incident light.