Title :
Photo-Induced DNA Double-Strand Breaks Monitored by Single-Molecule Observation: Protective Effect of Antioxidative Compounds
Author :
Yoshikawa, Yuko ; Hirose, Etsuko ; Sakai, Emi ; Ikawa, Chiharu
Author_Institution :
Int. Pacific Univ., Okayama
Abstract :
We have performed a real-time observation of photo-induced breakage on individual long DNA molecules stained by a cyanine dye, YOYO, under the intense illumination in solution. It is known that YOYO has ability to photo-cleave probed DNA by generating reactive oxygen species. We analyzed the kinetics of the breakage reaction in a quantitative manner. It was found that the breakage reaction is protected by antioxidative compounds; ascorbic acid and a water-soluble flavonoid, glucosyl-rutin. To interpret the kinetic process of the double-strand breakage reaction, a two-step mechanism is proposed.
Keywords :
DNA; biological effects of radiation; reaction kinetics; YOYO; antioxidative compounds; ascorbic acid; cyanine dye; glucosyl-rutin; photo-induced DNA double-strand breaks; photocleaving; reaction kinetics; water-soluble flavonoid; Chemical industry; DNA; Fluorescence; Kinetic theory; Lighting; Microscopy; Monitoring; Protection; Sugar refining; Time measurement;
Conference_Titel :
Micro-NanoMechatronics and Human Science, 2007. MHS '07. International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4244-1858-9
Electronic_ISBN :
978-1-4244-1858-9
DOI :
10.1109/MHS.2007.4420866
