Quantitative analysis of volumetric TPLSM data

Author

Ifarraguerri, Agustin ; Thompson, Beverly ; Tsai, Philbert S. ; Friedman, Beth ; Schaffer, Chris B. ; Lev-Ram, Varda ; Tsien, Roger Y. ; Squier, Jeff A. ; Kleinfeld, David

Author_Institution

Science Applications Int. Corp., Arlington, VA, USA

fYear

2004

fDate

15-18 April 2004

Firstpage

804

Abstract

This paper describes initial algorithm development in support of research on a new all-optical histology technique based on two-photon laser scanning microscopy (TPLSM) and laser tissue ablation. This new imaging modality makes it possible to obtain quantitative information that can be used to measure physiological parameters of interest. We present our initial image processing approach and volumetric rendering results. Two examples of useful quantitative parameters are presented: measurement of blood vessel volume fraction and cell counting.

Keywords

biomedical optical imaging; blood vessels; cellular biophysics; laser applications in medicine; medical image processing; optical microscopy; rendering (computer graphics); two-photon processes; all-optical histology; blood vessel volume fraction; cell counting; image processing; laser tissue ablation; volumetric two-photon laser scanning microscopy data; Biomedical imaging; Blood vessels; Fluorescence; Image reconstruction; Image segmentation; Laser ablation; Laser beam cutting; Laser theory; Microscopy; Rendering (computer graphics);

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on

Print_ISBN

0-7803-8388-5

Type

conf

DOI

10.1109/ISBI.2004.1398660

Filename

1398660