Title :
Using multi-element detectors to create optimal apertures in confocal microscopy
Author :
Davis, Brynmor J. ; Ünlü, M. Selim ; Swan, Anna K. ; Goldberg, Bennett B. ; Karl, W. Clem
Author_Institution :
Dept. of Electr. & Comput. Eng., Boston Univ., MA
Abstract :
A detection pinhole is used in confocal microscopy to reduce contributions from image planes outside of the focal plane. The size of this pinhole may be varied but the idea of a fixed circular aperture is ubiquitous. Here it is shown that an ideal detection aperture varies as a function of the spatial-frequency being imaged. A method for calculating such detection apertures is given, an example calculation is shown and a detector array is suggested as a means to approximate these varying detection apertures
Keywords :
optical images; optical microscopy; apertures; confocal microscopy; detection pinhole; multielement detectors; Apertures; Deconvolution; Detectors; Frequency; Image reconstruction; Microscopy; Optical noise; Sensor arrays; Shape; Signal to noise ratio;
Conference_Titel :
Lasers and Electro-Optics Society, 2005. LEOS 2005. The 18th Annual Meeting of the IEEE
Conference_Location :
Sydney, NSW
Print_ISBN :
0-7803-9217-5
DOI :
10.1109/LEOS.2005.1548121
