Developing metrology for tissue engineering: collinear optical coherence and confocal fluorescence microscopies

Author

Landis, Forrest A. ; Cicerone, Marcus T. ; Cooper, James A. ; Washburn, Newell R. ; Dunkers, Joy P.

Author_Institution

Polymers Div., Nat. Inst. of Stand. & Technol., Gaithersburg, MD, USA

fYear

2004

fDate

15-18 April 2004

Firstpage

1533

Abstract

We present a novel application of optical coherence microscopy (OCM) by combining it with confocal fluorescence microscopy (CFM) to gather simultaneous structural and functional information on tissue engineered medical products (TEMPs) in a registered fashion. In this work, we describe the collinear OCM and CFM instrument. We demonstrate the utility of this dual-mode technique for TEMPs by comparing the images of fluorescently stained osteoblasts cultured in a polymeric TEMP for 21 days and 10 weeks.

Keywords

biological techniques; fluorescence; optical microscopes; optical microscopy; polymers; tissue engineering; 10 weeks; 21 days; collinear optical coherence microscopy; confocal fluorescence microscopy; dual-mode technique; fluorescently stained osteoblasts; functional information; metrology; polymeric tissue engineered medical products; structural information; Biological tissues; Biomedical engineering; Biomedical optical imaging; Fluorescence; Metrology; Optical fiber polarization; Optical imaging; Optical interferometry; Optical microscopy; Tissue engineering;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on

Print_ISBN

0-7803-8388-5

Type

conf

DOI

10.1109/ISBI.2004.1398843

Filename

1398843