Title :
Developing metrology for tissue engineering: collinear optical coherence and confocal fluorescence microscopies
Author :
Landis, Forrest A. ; Cicerone, Marcus T. ; Cooper, James A. ; Washburn, Newell R. ; Dunkers, Joy P.
Author_Institution :
Polymers Div., Nat. Inst. of Stand. & Technol., Gaithersburg, MD, USA
Abstract :
We present a novel application of optical coherence microscopy (OCM) by combining it with confocal fluorescence microscopy (CFM) to gather simultaneous structural and functional information on tissue engineered medical products (TEMPs) in a registered fashion. In this work, we describe the collinear OCM and CFM instrument. We demonstrate the utility of this dual-mode technique for TEMPs by comparing the images of fluorescently stained osteoblasts cultured in a polymeric TEMP for 21 days and 10 weeks.
Keywords :
biological techniques; fluorescence; optical microscopes; optical microscopy; polymers; tissue engineering; 10 weeks; 21 days; collinear optical coherence microscopy; confocal fluorescence microscopy; dual-mode technique; fluorescently stained osteoblasts; functional information; metrology; polymeric tissue engineered medical products; structural information; Biological tissues; Biomedical engineering; Biomedical optical imaging; Fluorescence; Metrology; Optical fiber polarization; Optical imaging; Optical interferometry; Optical microscopy; Tissue engineering;
Conference_Titel :
Biomedical Imaging: Nano to Macro, 2004. IEEE International Symposium on
Print_ISBN :
0-7803-8388-5
DOI :
10.1109/ISBI.2004.1398843
