Multi-DNA extraction chip based on an aluminum oxide membrane integrated into a PDMS microfluidic structure

A novel microfluidic DNA extraction system was designed and fabricated using an AOM (aluminum oxide membrane) capture surface sandwiched between PDMS microfluidic channels in a parallel format. The completed system was tested with Lambda DNA mixed with the fluorescence dye SYBR Green I. Following extraction, the surface of AOM was examined with fluorescence microscopy while still embedded in the microfluidic system. Successful extraction and immobilization of DNA on the AOM was observed in almost every separation chamber. This microsystem has potential applications in high-throughput DNA extraction and analysis, with the capability to be integrated into polymer-based microfluidics systems.