Microfabricated fluorescence-activated cell sorter with hydrodynamic flow manipulation

Author

Bang, H. ; Chung, C. ; Kim, J.K. ; Chung, S. ; Lee, W.G. ; Han, D.-C. ; Chang, J.K.

Author_Institution

Sch. of Mech. & Aerosp. Eng., Seoul Nat. Univ., South Korea

fYear

2005

fDate

12-15 May 2005

Firstpage

13

Lastpage

14

Abstract

Presented is a novel flow manipulation and particle detection method for microfabricated fluorescence-activated cell sorter (μFACS). With hydrodynamic flow manipulation, our system guarantees fast and robust operation while sorting fluorescent particles. Moreover by detecting with synchronized imaging, we also developed an on-line calibration technique for accurate timing between detection and actuation.

Keywords

biological techniques; cellular biophysics; fluorescence; hydrodynamics; micromanipulators; fluorescence-activated cell sorter; fluorescent particle; hydrodynamic flow manipulation; microfabrication; on-line calibration technique; particle detection method; synchronized imaging; Calibration; Fluorescence; Focusing; Frequency synchronization; Hydrodynamics; Image motion analysis; Plastics; Robustness; Sorting; Valves; Lab-on-a-chip; PDMS; hydrodynamic flow manipulation; micro-FACS; plastic micromachining;

fLanguage

English

Publisher

ieee

Conference_Titel

Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on

Print_ISBN

0-7803-8711-2

Type

conf

DOI

10.1109/MMB.2005.1548370

Filename

1548370