Title :
Microfabricated fluorescence-activated cell sorter with hydrodynamic flow manipulation
Author :
Bang, H. ; Chung, C. ; Kim, J.K. ; Chung, S. ; Lee, W.G. ; Han, D.-C. ; Chang, J.K.
Author_Institution :
Sch. of Mech. & Aerosp. Eng., Seoul Nat. Univ., South Korea
Abstract :
Presented is a novel flow manipulation and particle detection method for microfabricated fluorescence-activated cell sorter (μFACS). With hydrodynamic flow manipulation, our system guarantees fast and robust operation while sorting fluorescent particles. Moreover by detecting with synchronized imaging, we also developed an on-line calibration technique for accurate timing between detection and actuation.
Keywords :
biological techniques; cellular biophysics; fluorescence; hydrodynamics; micromanipulators; fluorescence-activated cell sorter; fluorescent particle; hydrodynamic flow manipulation; microfabrication; on-line calibration technique; particle detection method; synchronized imaging; Calibration; Fluorescence; Focusing; Frequency synchronization; Hydrodynamics; Image motion analysis; Plastics; Robustness; Sorting; Valves; Lab-on-a-chip; PDMS; hydrodynamic flow manipulation; micro-FACS; plastic micromachining;
Conference_Titel :
Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on
Print_ISBN :
0-7803-8711-2
DOI :
10.1109/MMB.2005.1548370
