Application of a novel widefield surface plasmon microscope in binding assays

The present work investigates the capabilities of a novel widefield surface plasmon microscope (WSPR) and its potential applications in biology. Here in particular, we concentrate on imaging interfacial interactions between specific antibodies and the proteins laminin and fibronectin, both patterned onto prefabricated Au/Cr/glass substrates. These experiments are used to demonstrate the high sensitivity, good contrast and high lateral resolution (close to a micron) of the new system. These results indicate that our microscope may form the basis for a new generation of fast assay systems.