Title :
Investigation of temperature gradient effects on neurite outgrowth in nerve cells using a microfabricated heater structure
Author :
Jain, A. ; Ness, K.D. ; Fishman, H.A. ; Goodson, K.E.
Author_Institution :
Dept. of Mech. Eng., Stanford Univ., CA, USA
Abstract :
A novel heater structure capable of producing precisely controlled temperature gradients at cellular length scales is microfabricated. Protocols for successful culturing of RGC and PC12 cells on the heater surface are developed, and the heater structure is used to investigate the effect of spatial temperature gradients on neurite outgrowth in these cells. The results are discussed in the light of a recently developed theory for chemically-mediated thermotaxis.
Keywords :
biothermics; cellular biophysics; micromechanical devices; neurophysiology; PC12 cell culture; RGC cell culture; cell culture; cellular length scale; chemically-mediated thermotaxis; microfabricated heater structure; nerve cells; neurite outgrowth; protocols; temperature gradient effects; Aluminum; Biological control systems; Cells (biology); Chemicals; Heat engines; Heat transfer; Laboratories; Silicon; Temperature control; Temperature sensors; Microfabrication; neurite outgrowth; temperature gradient; thermotaxis;
Conference_Titel :
Microtechnology in Medicine and Biology, 2005. 3rd IEEE/EMBS Special Topic Conference on
Print_ISBN :
0-7803-8711-2
DOI :
10.1109/MMB.2005.1548487
