Title :
Manipulating behaviors of targeted single cells in vivo by using IR-LEGO
Author :
Suzuki, Motoshi ; Kamei, Yasuhiro ; Yuba, Shunsuke ; Takagi, Shin
Author_Institution :
Div. of Biol. Sci., Nagoya Univ., Nagoya, Japan
Abstract :
Methods for turning on/off gene expression at any desired time and place in vivo would be useful for analyzing various biological processes. We have developed a novel microscopic system utilizing an infrared laser, IR-LEGO (infrared-laser evoked gene operator), which is designed to deposit heat locally in living organisms. We have shown that IR-LEGO enables us to induce the heat shock response efficiently in targeted single cells of C. elegans worms, thereby driving expression of a transgene under the control of a heat shock promoter. By using IR-LEGO we attempted to rescue several mutant phenotypes of worms at the single-cell level. Diverse cell behaviors including differentiation and migration of target cells can be manipulated by gene induction mediated by IR-LEGO. Our results showed that IR-LEGO can be used to manipulate cell-autonomous as well as cell-nonautonomous behaviors, further confirming that irradiation using IR-LEGO has no harmful effects on the targets. Thus, IR-LEGO serves as valuable tools for manipulating biological processes in living organisms.
Keywords :
biological effects of laser radiation; cellular biophysics; cellular effects of radiation; genetics; infrared sources; microorganisms; C. elegans worms; IR-LEGO; differentiation; infrared-laser evoked gene operator; migration; targeted single cells; transgene expression; Biological processes; Electric shock; Gene expression; In vivo; Infrared heating; Microscopy; Optical design; Organisms; Temperature control; Turning;
Conference_Titel :
Micro-NanoMechatronics and Human Science, 2009. MHS 2009. International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4244-5094-7
Electronic_ISBN :
978-1-4244-5095-4
DOI :
10.1109/MHS.2009.5351899