Title :
Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter
Author :
Teramoto, Jun ; Yamanishi, Yoko ; Magdy, El-Shimy H. ; Hasegawa, Akiko ; Nakajima, Masahiro ; Shimada, Tomohiro ; Arai, Fumihito ; Fukuda, Toshio ; Ishihama, Akira
Author_Institution :
Dept. of Frontier Biosci., Hosei Univ., Tokyo, Japan
Abstract :
For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture within the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of promoter activity. Here we applied this newly developed system for measurement of the activity and regulation of E. coli gcl promoter.
Keywords :
biological techniques; cellular biophysics; microorganisms; Escherichia coli gcl promoter; cell chip; gelation; promoter activity; single live-bacterial cell assay; Bioinformatics; Genomics; Glass; Indium tin oxide; Lithography; Microelectrodes; Microorganisms; Polymers; RNA; Semiconductor device measurement;
Conference_Titel :
Micro-NanoMechatronics and Human Science, 2009. MHS 2009. International Symposium on
Conference_Location :
Nagoya
Print_ISBN :
978-1-4244-5094-7
Electronic_ISBN :
978-1-4244-5095-4
DOI :
10.1109/MHS.2009.5351949
