Functional analysis of a chromosomal passenger protein, Borealin, by multiphoton excitation-evoked chromophore-assisted laser inactivation

Borealin, one of the chromosomal passenger proteins, is reported to play important roles in the mitotic process. To analyze precise functions of Borealin in the mitotic phase, we applied the method of multiphoton excitation-evoked chromophore-assisted laser inactivation. Although further studies are needed, our results suggest that Borealin is required for mitosis, as previously reported. These results provide insights into understanding the functions of chromosomal passenger proteins during mitosis.