Realization of auto-focusing and tracking in the confocal LIF system

Improving the detect efficiency of confocal LIF device by using auto-focusing and tracking method, based on image processing. The Laplacian sharpness-evaluation-function and three image difference methods were used to calculate relative position between lens and object in the LIF system and implement auto-focusing and tracking. The experimental results shown that Laplacian sharpness-evaluation-function as evaluation function could give optimal focus point. Take coarse-to-precise method to satisfying the both requirement of accuracy and velocity in the auto-focusing.