Title :
Low-frequency dynamics of functional proteins studied by terahertz time-domain spectroscopy
Author :
Yamamoto, Naoki ; Zhang, Feng ; Tamura, Atsuo ; Tominag, Keisuke
Author_Institution :
Mol. Photoscience Res. Center, Kobe Univ., Kobe, Japan
Abstract :
We applied terahertz time-domain spectroscopy on myosin II subfragment 1 (S1) to monitor temperature and hydration dependence of its low-frequency dynamics. In the absorption spectra of both apo and holo S1 (ATP-free and bound form, respectively), so-called dynamical transition was observed at around 190 K only when they are hydrated.
Keywords :
DNA; monitoring; proteins; solvation; terahertz spectroscopy; ATP-free; DNA; absorption spectra; apo S1; bound form; dynamical transition; functional protein low-frequency dynamic; holo S1; hydration dependence; myosin II S1; myosin II subfragment1; temperature monitoring; terahertz time-domain spectroscopy; Absorption; Dynamics; Proteins; Spectroscopy; Temperature dependence; Temperature measurement; Time domain analysis;
Conference_Titel :
Infrared, Millimeter and Terahertz Waves (IRMMW-THz), 2011 36th International Conference on
Conference_Location :
Houston, TX
Print_ISBN :
978-1-4577-0510-6
Electronic_ISBN :
2162-2027
DOI :
10.1109/irmmw-THz.2011.6104944
