Direct Deformation of DNA Using Intense Burst RF Electric Field

Author

Nomura, Naoki ; Abe, S. ; Uchida, I. ; Abe, K. ; Koga, H. ; Katsuki, S. ; Namihira, T. ; Akiyama, H. ; Takano, H. ; Abe, S.-I.

Author_Institution

Dept. of Electr. Eng. & Comput. Sci., Kumamoto Univ.

fYear

2006

fDate

14-18 May 2006

Firstpage

490

Lastpage

493

Abstract

This paper describes the effect of intense burst RF electric fields (IBRFFs) on DNA in living mammalian cells. Chinese hamster ovary (CHO) cells were placed between two parallel plane electrodes and exposed to the IBRFF with a MHz range frequency and an electric field strength of a couple of kV/cm. The IBRFF duration was fixed to 100 ps, which was sufficiently short so as to not raise the temperature of suspension media of the cell due to Joule heating. A single cell electrophoresis analysis, comet assay method, was employed to investigate the reaction of the intracellular DNA to the lBRFF. The experiment shows the MHz range and 2kV/cm IBRFF physically introduces the fragmentation to intracellular DNA

Keywords

DNA; bioelectric phenomena; biological effects of fields; biological organs; biological techniques; biomechanics; biomolecular effects of radiation; cellular effects of radiation; deformation; electrophoresis; Chinese hamster ovary cells; comet assay method; direct DNA deformation; electric field strength; intense burst RF electric field effects; intracellular DNA fragmentation; intracellular DNA reaction; living mammalian cells; parallel plane electrodes; single cell electrophoresis analysis; suspension media; Biology; Biomembranes; Cells (biology); DNA; Electric fields; Electrodes; Electrokinetics; Nanobioscience; Radio frequency; Temperature;

fLanguage

English

Publisher

ieee

Conference_Titel

Power Modulator Symposium, 2006. Conference Record of the 2006 Twenty-Seventh International

Conference_Location

Arlington, VA

Print_ISBN

1-4244-0018-X

Electronic_ISBN

1-4244-0019-8

Type

conf

DOI

10.1109/MODSYM.2006.365291

Filename

4216243