A continuous optically-induced cell electroporation device with on-chip medium exchange mechanisms

We present a new design for continuous optically-induced electroporation (OIE) on a microfluidic device, which is capable of replacing culture medium and electroporation buffer in a seamless fashion. The seamless on-chip integration of medium exchange mechanisms and optically-induced electroporation device could avoid critical issues such as cell losses and cell damage during manual operation in the traditional centrifuge system, and is therefore suitable for handling small or rare cell population. Furthermore, the survival rate of the cells could be greatly improved due to this fast, automatic procedure.