Title :
Development a Risk Assessment Method of Brachyspira Hyodysenteriae in Pig Farms
Author :
Yahui, Kou ; Hengan, Wang ; Yaxian, Yan ; Jian, Wang ; Peihong, Liu
Author_Institution :
Sch. of Agric. & Biol., Key Lab. of Veterinary Biotechnol., Shanghai Jiao Tong Univ., Shanghai, China
Abstract :
Brachyspira hyodysenteriae is a one of important pathogen of Swine dysentery. This study, we established a indirect ELISA using the expressed and purified His6-Bhlp30.7 protein as coating antigen to detect pig sera antibodies to Bhlp30.7. All the parameters of ELISA were optimized. The positive and negative sera were detected using this ELISA. And the cut-off value was determined 4.0 through comparing the results of positive and negative sera. The indirect ELISA can be used to investigate the infected level of Brachyspira hyodysenteriae in pig farm group not in individual.
Keywords :
farming; microorganisms; proteins; risk management; Brachyspira Hyodysenteriae; coating antigen; expressed His6-Bhlp30.7 protein; indirect ELISA; negative sera; pathogen; pig farms; pig sera antibodies; positive sera; purified His6-Bhlp30.7 protein; risk assessment method development; swine dysentery; Biotechnology; Coatings; Diseases; Educational institutions; Proteins; Risk management; Strain; Brachyspira hyodysenteriae; Cut-off value; ELISA; Risk assessment;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.136
