Development of a GC-MS Method for the Simultaneous Determination of Amitraz in Fish Muscle

Author

Le, Tao ; Li, Bin ; He, Hong-qiu ; Chen, Yong ; Niu, Xiao-dong

Author_Institution

Chongqing Center for Biomedicines & Med. Equipments, Chongqing Acad. of Sci. & Technol. Chongqing, Chongqing, China

fYear

2012

fDate

28-30 May 2012

Firstpage

144

Lastpage

148

Abstract

An analytical procedure based on capillary GC-MS was developed for the quantification and characterization of 2, 4-dimethylaniline and amitraz in fish muscle. It contained an n-hexane/methanol extraction step using accelerated solvent extraction (ASE), and a selected ion monitoring (SIM) detection system of capillary GC-MS. The ASE parameters were optimized (P = 120 bar, T = 60°C) and GC-MS condition was validated after the mass fragments selection for 2, 4-dimethylaniline and amitraz quantification in fish muscle. The detection limits for these two analytes were 2 and 5 μg/kg, respectively. The recoveries at the spiked levels from 5 to 20 μg/kg ranged from 71% to 102%.

Keywords

biochemistry; chemical products; chromatography; mass spectroscopy; molecular biophysics; muscle; organic compounds; toxicology; zoology; 2,4-dimethylaniline; accelerated solvent extraction; amitraz; capillary GC-MS method; fish muscle; gas chromatography; mass spectroscopy; methanol extraction; n-hexaneextraction; selected ion monitoring detection; simultaneous determination; Acceleration; Compounds; Marine animals; Methanol; Muscles; Solvents; USA Councils; 2; 4-dimethylaniline; Accelerated solvent extraction; Amitraz; Fish muscle; Gas chromatography-mass spectrometry;

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on

Conference_Location

Macau, Macao

Print_ISBN

978-1-4577-1987-5

Type

conf

DOI

10.1109/iCBEB.2012.138

Filename

6245077