Impedimetric PSA Immunosensor: Prostate Specific Antigen Biosensor

Regular check of blood prostate specific antigen (PSA) level is very important as it is related to prostate cancer. Hence, this study aims to build up a novel impedimetric PSA immumosensor (PSA-IMS) for accurate measurement of PSA. The impedimetric PSA-IMS was fabricated with PSA antibody immobilized on screen-printed carbon electrodes. Impedance measurement at a specific frequency ranges (22.80 Hz - 2.57 kHz) showed that the PSA-IMS has an excellent response range (6.25 - 400 ng/mL), with r²>;0.9, which covers the normal physiological and pathological ranges of blood PSA levels. Intraclass correlation coefficient (ICC) showed that the PSA-IMS has excellent reliability and validit (ICC>;0.95). In conclusion, a simple and reliable PSA-IMS was developed and it is capable of precisely determining blood PSA levels in the range of pathological and normal physiological regions. in both the normal physiological and pathological ranges.