Title :
Purification of Polysaccharide from Photoautotrophic Cultivation of Nostoc flagelliforme Cells with Sector Radial Flow Chromatography
Author :
Dai, Yujie ; Chen, Nan ; Jia, Shiru ; Yuan, Nannan ; Tan, Ning ; Li, Jing
Author_Institution :
Key Lab. of Ind. Fermentation Microbiol., Univ. of Sci. & Technol., Tianjin, China
Abstract :
This paper studied the application of sector radial flow chromatography (SRFC) filled with DEAE Sephadex CL-6B ion exchange resin to the separation and purification of polysaccharide of Nostoc flagelliforme. The effects of different factors on the separation efficiency were examined, and the optimal separation conditions were determined. The comparison of the separation efficiency of the sector radial chromatography with the cylindrical one also has been conducted. The results showed that the optimal separation conditions were 1.0 mg/ml of the sampling concentration, 0.5 ml/min of the elution velocity and 0.2 mol/l of NaCl elution. The purity of polysaccharides of Nostoc flagelliforme rise to 97.7% from 12.9%.
Keywords :
biotechnology; chromatography; microorganisms; purification; separation; sodium compounds; DEAE Sephadex CL-6B ion exchange resin; NaCl; Nostoc flagelliforme cell; SRFC; cylindrical chromatography; elution velocity; optimal separation condition; photoautotrophic cultivation; polysaccharide purification; polysaccharide purity; polysaccharide separation; sampling concentration; sector radial flow chromatography; separation efficiency; sodium chloride elution; Biotechnology; Instruments; Liquids; Mobile communication; Purification; Resins; Nostoc flagelliforme; polysaccharide; purification; sector radial flow chromatography;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.321
