The Effect of CCl4-induced Acute Liver Injury on the ADAM8 Expression in the Mice

To study the the expression of ADAM8 (disintegrin and metalloprotease 8) during acute liver failure of mice induced by CCl₄. The mice model of acute liver failure was established by CCl₄ (0.1% in olive oil, 0.1mL/10g) abdominal injection. The liver pathological changes were observed at 0, 3, 6, 12, 24, 30, 36, 42, 48, 54h after CCl₄ injection respectively. The expressions of ADAM8 protein were determined by Western blot. Compared with the control, the liver tissue structures were all harmed from 3h to 54h after CCl₄ injection at the different level, especially at 24h, reached to the highest level. The expression of ADAM8 protein was significantly changed and reached the lowest level at 24h and then gradually increased, but did not recover to the normal level. The expression of ADAM8 protein were significantly changed and probably play an important roles in the acute liver damage by CCl₄.