The Electrochemical Impedance Recognition for Two Heme Proteins on dsDNA/PGA/GCE

Author

Zhang, Rongrong ; Xie, Chunqing ; Zhu, Yayun ; Pan, Liangjun ; Yang, Changying

Author_Institution

Coll. of Chem. & Life Sci., Three Gorges Univ., Yichang, China

fYear

2012

fDate

28-30 May 2012

Firstpage

925

Lastpage

928

Abstract

A DNA-modified electrode was fabricated by the way of electrode modifying, namely the polymerisation-covalently bonding, for studying the recognition of DNA to heme protein. Electrochemical Impedence Resistance (EIS) showed that electron transfer resistance increased with the adding of Hemoglobin (Hb) and Cytochrome c (Cyt c). Reaction constant of Hb and DNA is K=1.13×10⁵ L-mol^-1, and K=4.08×10⁵ L-mol^-1 for Cyt c, indicating that DNA-modified electrode can well recognize Hb and Cyt c.

Keywords

DNA; biochemistry; biosensors; charge exchange; electrochemical electrodes; electrochemical impedance spectroscopy; electrochemical sensors; molecular biophysics; polymerisation; proteins; reaction rate constants; DNA modified electrode; DNA recognition; EIS; cytochrome c; dsDNA-PGA-GCE; electrochemical impedance recognition; electrochemical impedence resistance; electrochemical impedence spectroscopy; electrode modifying; electron transfer resistance; glassy carbon electrode; heme proteins; hemoglobin; poly(glutamic acid); polymerisation-covalent bonding; reaction constant; DNA; Electrodes; Electronics packaging; Impedance; Proteins; Surface impedance; Surface resistance; Cytochrome c (Cyt c); DNA-Modified electrodes; Electrochemical Impedence Resistance (EIS); Hemoglobin (Hb);

fLanguage

English

Publisher

ieee

Conference_Titel

Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on

Conference_Location

Macau, Macao

Print_ISBN

978-1-4577-1987-5

Type

conf

DOI

10.1109/iCBEB.2012.13

Filename

6245273