Title :
Analysis of Sonoporation on Cell Membrane by Scanning Electron Microscopy
Author :
Yu, Hao ; Chen, Siping
Author_Institution :
Biomed. Eng. Dept., Zhejiang Univ., Hangzhou, China
Abstract :
Sonoporation is an important drug/gene delivery technology in drug/gene targeting therapy. In this article, the fibroblast cell and red blood cell suspensions were added contrast agent micro bubbles and sonicated by continues low-intensity ultrasound, and then the pores on cell membrane were detected by scanning electron microscopy. It was found that the sonoporation on cell membrane was positive correlation with the ultrasound exposure power and ultrasound exposure time. And it was found that the sonoporation was related with cell membrane elastic characteristics. It provided experiment basement for the qualitative and quantitative research on sonoporation.
Keywords :
biomechanics; biomedical ultrasonics; biomembranes; blood; bubbles; cellular biophysics; drugs; elasticity; genetics; scanning electron microscopy; suspensions; cell membrane elastic properties; cell membrane pores; continues low-intensity ultrasound; contrast agent microbubbles; drug-gene delivery technology; drug-gene targeting therapy; fibroblast cell suspension; red blood cell suspension; scanning electron microscopy; sonoporation analysis; ultrasound exposure power; ultrasound exposure time; Biomembranes; Drugs; Scanning electron microscopy; Suspensions; Ultrasonic imaging; Ultrasonic variables measurement; cell membrane; contrast agent microbubbles; sonoporation; ultrasound;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.70
