Title :
Cloning and Sequence Analysis of Rongchang Porcine Interferen-gamma Gene
Author :
Ding, Shan-shan ; Leng, Yong ; Song, Zhen-hui
Author_Institution :
Animal Med. Sci., Southwest Univ., Chongqing, China
Abstract :
Interferen-γ of Rongchang porcine was amplifed by RT-PCR with the total RNA extracted from the peripheral blood lymphocytes. The gene was sequenced after cloned into the vector pMD18-T and analyzed with molecular biological software. The results showed that the opening reading frame (ORF) of interferen-γ of Rongchang porcine consists of 501 nucleotides, encoding 166 amino acids, the front 23 amino acide is signal peptide. The homology of IFN-γ of rongchang porcine with chenghua porcine interferon is 99.8% at nucleotide level. The homology of susscrofa IFN-γ is 96.8%. the homology of rongchang porcine IFN-γ with that of camel, rabbit, horse, goat, cattle, gorilla and human is 84.8%, 71.8%, 77.6%, 60.7%, 84.0%, 69.5% and 70.3%. Phylogenetic analysis based on IFN-γ gene showed that rongchang porcine had the closest linkage to chenghua procine.
Keywords :
molecular configurations; proteins; proteomics; zoology; Chenghua porcine interferon; IFN-gamma homology; RNA extraction; RT-PCR; Rongchang porcine; amino acids; camel interferon; cattle interferon; goat interferon; gorilla interferon; horse interferon; human interferon; interferen-gamma gene cloning; interferen-gamma gene sequence analysis; molecular biological software; nucleotides; pMD18-T vector; peripheral blood lymphocytes; phylogenetic analysis; rabbit interferon; reverse transcription polymerase chain reaction; signal peptide; susscrofa IFN-gamma; Animals; Cloning; Educational institutions; Humans; Immune system; RNA; Sequences; Rongchang porcine; interferen-?; sequence analysis;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.2
