Construction of a Eukaryotic Expression Vector pEGFP-C1-BMP-2

It has been known that bone morphogenetic proteins not only play important role in bone development, but also induce tumorigenesis and promote tumor spread. However, the molecular mechanisms have not been elucidated. To investigate the mechanisms underlying the regulatory function of BMP-2 on cell migration, BMP-2 genetic fragment was amplified and introduced into the pEGFP-C1 vector. After being confirmed by restriction enzymes digestion analysis and DNA sequencing, the recombinant pEGFP-C1-BMP-2 plasmid was transfected into COS-7 cells. Western Blotting analysis and fluorescence microscopy were applied to detect the expression of BMP-2 on COS7 cell. The results indicated that the recombinant pEGFP-C1-BMP-2 was successfully constructed and effectively expressed in COS-7 cells.