Title :
Construction of Ginseng CS Gene RNA Interference Plant Expression Vector
Author :
Mu-yang He ; Hao-jie Cao ; Le Wang ; Shou-jing Zhao
Author_Institution :
Coll. of Biol. & Agric. Eng., Jilin Univ., Changchun, China
Abstract :
This study is through the Recombinant PCR reaction and restriction enzyme digestion connection to construct ginseng CS RNA interference plant expression vector, was transformed by heat into the Agro bacterium rhizogenes A4 to get CS RNA interference plant expression vector engineered bacteria, and lay the foundation for further induced transformation of ginseng explants.
Keywords :
RNA; biochemistry; biothermics; cellular biophysics; enzymes; genetics; microorganisms; molecular biophysics; Agro bacterium rhizogenes A4; CS RNA interference plant expression vector; ginseng; heat transformation; recombinant PCR reaction; restriction enzyme digestion connection; Biochemistry; Biotechnology; DNA; Interference; Microorganisms; RNA; Vectors; CS; Engineering bacteria; Ginsenoside; RNAi plant expression vector;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.115
