Title :
Detection of Salmonella choleraesuis by Loop-mediated Isothermal Amplification
Author :
Yin, Huan ; Li, Qi ; Liu, Lieju ; Chen, Jiangyuan ; Wang, Lan ; Liu, Zhiguo
Author_Institution :
Coll. of Biol. & Pharm. Eng., Wuhan Polytech. Univ., Wuhan, China
Abstract :
Objective; To detect Salmonella choleraesuis rapidly, Loop-mediated isothermal amplification (LAMP) method was developed. Methods: According to fliC gene of S. choleraesuis flagellin protein, LAMP inner and outer primer sets were designed. Amplification reaction carried out within tens of minutes. Specificity of LAMP primers were validated by as-saying 10 different strains. Sensitivity of LAMP method was evaluated by serial gradient dilution of S. choleraesuis cultures. LAMP used for the detection of simulated meat samples. Results: S. choleraesuis LAMP method was rapid and specific. The detection limits of LAMP assay were 1.33×101 CFU/mL for pure cultures and 2.0×101 CFU/mL for simulated meat samples. Conclusion: LAMP method is a rapid and feasible method for the detection of S. choleraesuis.
Keywords :
biochemistry; biological techniques; biotechnology; food technology; genetics; microorganisms; molecular biophysics; proteins; LAMP inner primer sets; LAMP method; LAMP outer primer sets; LAMP primer specificity; S. choleraesuis flagellin protein; amplification reaction; fliC gene; loop mediated isothermal amplification; rapid Salmonella choleraesuis detection; serial gradient dilution; simulated meat samples; Animals; DNA; Isothermal processes; Microorganisms; Polymers; Sensitivity; Strain; Loop-mediated isothermal amplification; Salmonella choleraesuis; detection; detection limit;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macau, Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.131
