Title :
Induction and Selection of Stenotrophomonas maltophilia DHHJ for Feather Degradation
Author :
Wu, Xiaoqian ; Wang, Jing ; Zhuang, Yuan ; Cao, Zhangjun ; Zhou, Meihua
Author_Institution :
Dept. of Environ. Sci. & Eng., Donghua Univ., Shanghai, China
Abstract :
Stenotrophomonas maltophilia DHHJ, a new feather-degrading bacterium, was subjected to mutation by ultraviolet light for 90 s. The mutant L2 was selected due to its greater activity in comparison with the wild strain. After incubation at 40°C for 6 days, the feathers degraded by mutant L2 were 1.3-fold more than S. maltophilia DHHJ, and the keratinase was secreted. After cultivation for 6 days in 5 L fermenter with 50% air saturation, the rate of feather degradation was 92.3% and the activity of keratinase produced was 8.6 U/mL by mutant L2. The results proved that mutant L2 had high genetic stability and could be possibly used in the industrial processes.
Keywords :
cellular biophysics; enzymes; genetics; microorganisms; molecular biophysics; Stenotrophomonas maltophilia DHHJ; air saturation; feather-degrading bacterium; genetic stability; incubation; industrial processes; keratinase activity; mutant L2; temperature 40 degC; time 6 d; time 90 s; ultraviolet light mutation; Degradation; Feathers; Microorganisms; Proteins; Radiation effects; Strain; Ultraviolet light; degradation; feather; mutation;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.248