Preparation of Immobilized EGFR Micro-reactor Based on CE

As the member of receptor tyrosine kinase family, epidermal growth factor receptor (EGFR) is closely related to the growth of tumor. Thus, it has become a significant and potential target molecule for the screening of anti-tumor drug. In this study, a new immobilized EGFR micro-reactor was established for fast screening EGFR inhibitors. Enzyme was immobilized into the inner wall of capillary with three chemical modification methods. Combined with capillary electrophoresis technique, the addressable immobilized EGFR micro-reactor could easily separate and capture EGFR inhibitors from the complex mixtures with high selectivity. The performance of the micro-reactor was studied with Gefitinib, which showed good recognization ability. The influence on stability and activity of the immobilized enzyme prepared by three methods was further investigated. It was demonstrated that the layer-by-layer assembling method was the optimized one, which showed both good stability and activity. It is expected that this screening platform would be widely used in the discovery of the new protein inhibitors as a convenient and universal method.