Title :
Specific Immunity Elicited by Cross-link Complex Alpha-fetoprotein and Glycoprotein 96
Author :
Wang, Xiaoping ; Lin, Huanping ; Wang, Qiaoxia ; Gu, Yan ; Xu, Yi
Author_Institution :
Lab. of Mol. Pathology, Shaanxi Univ. of Chinese Med., Xianyang, China
Abstract :
Alpha-fetoprotein (AFP) is an oncofetal antigen during hepatocellular carcinoma (HCC) development which could lead to weak reproducible antitumor immunity, and may act as a target for cancer therapy. Therefore, it is imperative to enhance its immunogenicity and develop therapeutic vaccines to eliminate AFP-expressing tumors. In this study, by way of glutaraldehyde cross-linking, we constructed a potential therapeutic protein vaccine, gp96/AFP. Our results showed that AFP and gp96 induced significant enhancement in specific AFP CD8+ T cells response and distinct cytotoxic antitumor reaction against AFP-expressing tumors. Primimg mice with the reconstructed vaccine, we elicited robust strong protective immunity. Our study suggests that tumor vaccination through cross-linking tumor antigen and gp96 is an encouraging method for cancer immunotherapy.
Keywords :
cancer; cellular biophysics; molecular biophysics; proteins; toxicology; tumours; AFP CD8+ T cell response; alpha-fetoprotein-glycoprotein 96 cross-link complex; antitumor immunity; cancer immunotherapy; cytotoxic antitumor reaction; glutaraldehyde cross-linking; gp96-AFP expressing tumors; hepatocellular carcinoma; oncofetal antigen; protein vaccine; therapeutic vaccines; Cancer; Immune system; Medical treatment; Mice; Proteins; Tumors; Vaccines; alpha-fetoprotein (AFP); cross-link vaccine; glycoprotein96 (gp96); immunotherapy;
Conference_Titel :
Biomedical Engineering and Biotechnology (iCBEB), 2012 International Conference on
Conference_Location :
Macao
Print_ISBN :
978-1-4577-1987-5
DOI :
10.1109/iCBEB.2012.361
