Title :
Combining gene expression and function in a spatially localized approach
Author :
Zacharaki, Evangelia I. ; Skoura, Angeliki ; Smith, Desmond J. ; Faro, Scott H. ; An, Li ; Megalooikonomou, Vasileios
Author_Institution :
Dept. of Comput. Eng. & Inf., Univ. of Patras, Patras, Greece
Abstract :
The integration of gene expression datasets with gene function information provides valuable insights in unraveling the molecular mechanisms of the brain. In this paper, gene expression maps, acquired by the technique of voxelation, are analyzed using an atlas-based framework and the extracted spatial information is employed to organize genes in significant clusters. Gene function enrichment analysis of clusters enabled exploration of the relationships among brain regions, gene expression and gene function. In addition, the analysis revealed new function categories, for example biological process for the gene Pbx3, cellular component and biological process for the gene Ppplrlb. Our work confirms the hypothesis that genes of similar spatial expression patterns display similar functions indicating that our methodology could assist in the function identification of unannotated genes.
Keywords :
bioinformatics; brain; genetics; knowledge engineering; molecular biophysics; neurophysiology; pattern classification; Pbx3 gene; Ppplrlb gene; atlas based framework; brain molecular mechanisms; gene expression datasets; gene expression maps; gene function enrichment analysis; gene function information; spatially localized approach; voxelation; Biological processes; Educational institutions; Focusing; Gene expression; Mice; Ontologies; Prototypes; brain mapping; gene expression maps; gene function; gene ontology; voxelation;
Conference_Titel :
Bioinformatics and Biomedicine (BIBM), 2012 IEEE International Conference on
Conference_Location :
Philadelphia, PA
Print_ISBN :
978-1-4673-2559-2
Electronic_ISBN :
978-1-4673-2558-5
DOI :
10.1109/BIBM.2012.6392675
