Title :
In-situ single cell electroporation using optoelectronic tweezers
Author :
Valley, Justin K. ; Hsu, Hsan-Yin ; Ohta, Aaron T. ; Neale, Steven ; Jamshidi, Arash ; Wu, Ming C.
Author_Institution :
Dept. of Electr. Eng. & Comput. Sci., California Univ., Berkeley, CA
Abstract :
Optoelectronic tweezers are used to achieve light-induced, in-situ electroporation of HeLa cells. By controlling electrical bias, patterned light induces either single cell movement or electroporation. Fluorescent dye and dielectrophoretic response are used to monitor electroporation.
Keywords :
biological techniques; biomembrane transport; electrophoresis; radiation pressure; HeLa cells; dielectrophoretic response; electrical bias control; fluorescent dye; in-situ single cell electroporation; light-induced electroporation; optoelectronic tweezers; single cell movement; Biomembranes; Cells (biology); Conductivity; Dielectrophoresis; Glass; Indium tin oxide; Lighting control; Optical devices; Optical films; Throughput;
Conference_Titel :
Optical MEMs and Nanophotonics, 2008 IEEE/LEOS Internationall Conference on
Conference_Location :
Freiburg
Print_ISBN :
978-1-4244-1917-3
Electronic_ISBN :
978-1-4244-1918-0
DOI :
10.1109/OMEMS.2008.4607835
