Generation of Oxidative Stress in Cells by Localized Laser Radiation

Author

He, Hao ; Kong, Siu-Kai ; Lee, Rebecca Kit-Ying ; Chan, Kam Tai

Author_Institution

Dept. of Electron. Eng., Chinese Univ. of Hong Kong, Hong Kong

fYear

2008

fDate

8-11 Dec. 2008

Firstpage

1

Lastpage

3

Abstract

Localized laser beams had been observed to generate reactive oxygen species (ROS) in human hepatocellular carcinoma cells (HepG2). The oxidative stress and thermal effect generated depended on the nature of the laser beam used. It was found that the ROS-induced fluorescence signal caused by femtosecond (fs) laser pulses were much smaller than that due to continuous-wave laser radiation around 1550nm. This implies fs laser pulses are relatively harmless to cells.

Keywords

biological effects of laser radiation; cellular biophysics; fluorescence; high-speed optical techniques; laser applications in medicine; ROS-induced fluorescence signal; continuous-wave laser radiation; femtosecond laser pulses; human hepatocellular carcinoma cells; localized laser radiation; oxidative stress; reactive oxygen species; thermal effect; Cells (biology); Fiber lasers; Fluorescence; Humans; Laser beams; Microscopy; Optical pulses; Power lasers; Stress; Ultrafast optics;

fLanguage

English

Publisher

ieee

Conference_Titel

PhotonicsGlobal@Singapore, 2008. IPGC 2008. IEEE

Conference_Location

Singapore

Print_ISBN

978-1-4244-3901-0

Electronic_ISBN

978-1-4244-2906-6

Type

conf

DOI

10.1109/IPGC.2008.4781389

Filename

4781389