Title :
Non-destructive real-time imaging of cell morphology for tissue-engineering applications
Author :
Hofmann, Matthias C. ; Whited, Bryce M. ; Goldstein, Aaron S. ; Freeman, Joseph W. ; Rylander, Marissa Nichole ; Rylander, Chris ; Furth, Mark ; Wang, Ge ; Soker, Shay ; Xu, Yong
Author_Institution :
Bradley Dept. of Electr. & Comput. Eng., Virginia Polytech. Inst. & State Univ., Blacksburg, VA, USA
Abstract :
A major barrier for progress in regenerative medicine is the inability to observe the process of tissue regeneration non-destructively, in real time, and with cellular level resolution. In order to overcome this difficult challenge, we have proposed and developed an imaging-bioreactor system based on fiber micro-devices. Our system is based on directly incorporating multiple hollow core fibers (HCFs) into a biocompatible tissue scaffold. By inserting fiber-based micro-mirrors into the HCFs and applying a straightforward scanning-reconstruction algorithm, we have demonstrated an imaging resolution of ~200 μm through an optically opaque scaffold.
Keywords :
biomedical optical imaging; bioreactors; cellular biophysics; image reconstruction; image resolution; medical image processing; micromirrors; optical fibres; tissue engineering; biocompatible tissue scaffold; cell morphology; cellular level resolution; fiber microdevices; fiber-based micromirrors; imaging-bioreactor system; multiple hollow core fibers; nondestructive real-time imaging; optically opaque scaffold; regenerative medicine; straightforward scanning-reconstruction algorithm; tissue regeneration; tissue-engineering; Biomedical imaging; Fluorescence; Microwave integrated circuits; Optical fibers; Tissue engineering; cells; scaffold; tissue engineering;
Conference_Titel :
Information Optics (WIO), 2011 10th Euro-American Workshop on
Conference_Location :
Benicassim
Print_ISBN :
978-1-4577-1227-2
Electronic_ISBN :
978-1-4577-1225-8
DOI :
10.1109/WIO.2011.5981465
