Chitosan-mediated Patterned Viral Nanotemplate Assembly onto Inorganic Substrates through Nucleic Acid Hybridization

Author

Yi, Hyunmin ; Ghodssi, Reza ; Rubloff, Gary W. ; Culver, James N.

Author_Institution

Dept. of Mater. Sci. & Eng., Maryland Univ., College Park, MD

fYear

2006

fDate

38899

Firstpage

1

Lastpage

2

Abstract

We present patterned assembly of tobacco mosaic virus (TMV) through hybridization of its own genomic information with probe DNA assembled onto electrodeposited chitosan scaffold. Chitosan´s unique pH-responsive and chemically reactive properties allow facile biomolecule assembly onto conductive surfaces from aqueous environment. Genetically modified TMV serves as a potent nanotemplate for covalent conjugation of fluorescent markers or nanoparticles. This work represents a significant step toward assembling biological components for devices in biomedical applications

Keywords

DNA; cellular biophysics; genetics; microorganisms; molecular biophysics; nanobiotechnology; pH; polymers; chemically reactive properties; chitosan-mediated patterned viral nanotemplate assembly; electrodeposited chitosan scaffold; facile biomolecule assembly; fluorescent markers; genomic information; inorganic substrates; nanoparticles; nucleic acid hybridization; pH-responsive properties; probe DNA; properties devices; tobacco mosaic virus; Assembly; Bioinformatics; Chemicals; DNA; Fluorescence; Genomics; Molecular biophysics; Nanobioscience; Nanoparticles; Probes;

fLanguage

English

Publisher

ieee

Conference_Titel

Life Science Systems and Applications Workshop, 2006. IEEE/NLM

Conference_Location

Bethesda, MD

Print_ISBN

1-4244-0277-8

Electronic_ISBN

1-4244-0278-6

Type

conf

DOI

10.1109/LSSA.2006.250423

Filename

4015824